Extraction of Nuclear Proteins with Increased DNA Binding Activity

Extraction of nuclear proteins with increased DNA binding activity.

We have developed a method for the rapid extraction of nuclear proteins from cultured cells. The ammonium sulfate method described here extracts larger quantities of proteins that retain DNA binding activity than the modified Dignam method and another popular method used for the extraction of transcription factors. The ammonium sulfate method is rapid and can be used to process a large number o...

DNA-binding properties of nuclear matrix proteins.

Mouse nuclear matrix proteins, examined by a filter assay, were found to bind to DNA. There was no preference for homologous mouse compared to heterologous E. coli DNA. Competition assays showed a preference for AT-rich DNA and of the 4 single-stranded homopolymers there was a preference for poly(dT). These observations are consistent with the possibility that the matrix may play a role in the ...

Control of nuclear factor-kappa B DNA-binding activity by inhibitory proteins containing ankyrin repeats.

Patterns of nuclear fluorescence and DNA-binding activity.

Sera from patients with systemic lupus erythematosus (SLE) and other connective tissue diseases have been demonstrated to contain a variety of antinuclear antibodies. Antibodies to native DNA appear to be present only in sera from patients with clinically active SLE. These antibodies have been detected by a variety of techniques, such as precipitation in agar (Seligmann, 1957), complement fixat...

Analysis of epidermal proteins for DNA-binding activity.

A group of DNA-binding proteins from the soluble extract of newborn rat epidermis have been separated by chromatography using DNA-cellulose columns. The electrophoretogram of the DNA-binding proteins eluted from a single stranded DNA-cellulose column shows five major proteins of molecular weights ranging between 25K to 40K. Both the epidermal protein filaggrin and most keratins, except two high...